產(chǎn)地 | 美國 |
品牌 | Zymo |
貨號 | D5460 |
用途 | 亞硫酸氫鹽測序 |
包裝規(guī)格 | 24 Preps |
純度 | % |
Cat # | Name | Size |
D5460 | Zymo-Seq RRBS Library Kit (24 preps) | 24 Preps |
D5461 | Zymo-Seq RRBS Library Kit (48 preps) | 48 Preps |
簡單的工作流程:在2個(gè)小時(shí)的動手時(shí)間內(nèi)準(zhǔn)備,減少亞硫酸氫鹽測序(RRBS)庫處理時(shí)間。
低輸入:從10ng的基因組DNA≥NGS庫。
準(zhǔn)確且可重復(fù):無偏甲基化調(diào)用和可重復(fù)的CpG覆蓋。
Zymo-Seq RRBS Library Kit是一體式 RRBS 庫準(zhǔn)備套件,用于生成具有簡化工作流程的 NGS 庫,減少亞硫酸氫鹽測序 (RRBS)文庫制備時(shí)間。這種一體式RRBS庫制備套件允許在基因組CpG富產(chǎn)區(qū)的單核苷酸分辨率下進(jìn)行DNA甲基化分析。此 Zymo-Seq RRBS 庫套件是理想的 RRBS 庫準(zhǔn)備套件,用于生成涵蓋 ≥所有 CpG 孤島、運(yùn)動體和基因體的重要百分比(如果使用人類基因組 DNA,則涵蓋 70%)的庫,同時(shí)大大降低了測序成本,使其成為高通量篩選和比較實(shí)驗(yàn)的的理想之選。
技術(shù)規(guī)格:
所需設(shè)備 | 微離心器,帶加熱蓋的熱循環(huán)器 |
輸入 | 10 ~ 500 ng的基因組 DNA |
示例源 | 純化基因組DNA。DNA應(yīng)不含酶抑制劑,可懸浮在水中、TE或低鹽緩沖液中。低 260/280 或 260/230 比率的 DNA 應(yīng)在使用基因組 DNA 清潔和濃縮器(Cat.D4010).對于來自低CpG密度的物種的樣品,不建議使用此協(xié)議。 |
排序平臺兼容 | 庫與除HiSeq X系列以外的所有光照測序平臺兼容。 |
關(guān)鍵組分:
Cat # | Name | Size |
D4014 | DNA Clean & Concentrator-5 (Capped) | 200 Preps |
D4013 | DNA Clean & Concentrator-5 (Capped) | 50 Preps |
D3004-4-1 | DNA Elution Buffer | 1 ml |
D3004-4-10 | DNA Elution Buffer | 10 ml |
D3004-4-16 | DNA Elution Buffer | 16 ml |
D3004-4-4 | DNA Elution Buffer | 4 ml |
D3004-4-50 | DNA Elution Buffer | 50 ml |
W1001-1 | DNase/RNase-Free Water | 1 ml |
W1001-10 | DNase/RNase-Free Water | 10 ml |
W1001-30 | DNase/RNase-Free Water | 30 ml |
W1001-4 | DNase/RNase-Free Water | 4 ml |
W1001-6 | DNase/RNase-Free Water | 6 ml |
D4003-1-25 | DNA Binding Buffer | 25 ml |
D4003-1-L | DNA Binding Buffer | 50 ml |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml |
D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml |
D5001-3 | M-Binding Buffer | 20 ml |
D5002-3 | M-Binding Buffer | 80 ml |
D5040-3 | M-Binding Buffer | 250 ml |
D5001-4 | M-Wash Buffer | 6 ml |
D5002-4 | M-Wash Buffer | 24 ml |
D5007-4 | M-Wash Buffer | 36 ml |
D5040-4 | M-Wash Buffer | 72 ml |
D5005-3 | M-Binding Buffer | 30 ml |
D5006-3 | M-Binding Buffer | 125 ml |
D5030-5 | L-Desulphonation Buffer | 10 ml |
D5031-5 | L-Desulphonation Buffer | 40 ml |
D5046-5 | L-Desulphonation Buffer | 80 ml |
D5030-1 | Lightning Conversion Reagent | 1.5 ml |
D5032-1 | Lightning Conversion Reagent | 15 ml |
D5016 | E. coli Non-Methylated Genomic DNA | 5 μg/20 μl |
D5030T | EZ DNA Methylation-Lightning Kit | 10 Rxns. |
D5030 | EZ DNA Methylation-Lightning Kit | 50 Rxns. |
D5031 | EZ DNA Methylation-Lightning Kit | 200 Rxns. |
C1001-50 | Collection Tubes | 50 Pack |
C1001-500 | Collection Tubes | 500 Pack |
C1001-1000 | Collection Tubes | 1000 Pack |
C1004-50 | Zymo-Spin IC Columns | 50 Pack |
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