產(chǎn)地 | 美國Agilent 210519 |
品牌 | Agilent 210519 |
貨號 | 210519 |
保存條件 | -20度 |
英文名稱 | Agilent 210519 QuikChange Lightning 30 rxn |
保質(zhì)期 | 2年個月 |
agilent QuikChange Lighting 定點突變試劑盒 200515
QuikChange Lightning Multi Site-Directed Mutagenesis Kit - Academic, 10 Rxn
QuikChange Lightning 定點突變試劑盒 (Cat. 200515)
最初的QuikChange定點突變試劑盒省去了亞克隆到M13基于噬菌體載體和用于單鏈DNA的救援。這使得位點定向誘變研究簡單和可靠的,因為它允許寡核苷酸介導(dǎo)將位點特異性突變引入幾乎任何雙鏈質(zhì)粒DNA。XL版試劑盒是專門為大型高效突變(4-14 KB)或以其他方式難以誘變的質(zhì)粒模板和功能專為更高效的DNA復(fù)制和細(xì)菌轉(zhuǎn)化設(shè)計的部件。QuikChange多系統(tǒng)通過使用針對不同位點的寡核苷酸允許單輪誘變多個站點(最多5個)。?1個位點的突變效率大于80%;同時突變3個位點的效率大于 55%
?高效的和準(zhǔn)確的線性擴增最小化不希望出現(xiàn)的錯誤.
Agilent 210519 QuikChange Lightning 30 rxn
品牌: Agilent牌
QuikChange Lightning定點誘變試劑盒 包括了我們*的超級高保真的QuikChange Lightning聚合酶融合 。
使用了QuikSolution溶液來加強擴增
用我們的*效的XL-10 Gold 超級感受態(tài)細(xì)胞
下一代的加速實驗方案
新的QuikChange Lightning定點誘變試劑盒(下稱QuikChange)把我們的基因突變技術(shù)提升到新的高度。引入點突變、插入、或缺失的速度比我們原來的試劑盒要更快。適用特別優(yōu)化過的更快的酶,我們在保持高精確性、高效性、易用性的同時,我們讓實驗過程更快。我們的QuikChange Lightning酶與我們的PfuUltra有一樣的超高保真性能,但是是一種高前進性聚合酶,用來合并具有超高保真性能和快速延伸能力的誘變引物。另外,DpnI限制性內(nèi)切酶可以在5分鐘內(nèi)快速地將親代質(zhì)粒DNA降解。這些創(chuàng)新的酶只有在我們的QuikChange試劑盒中才*。
確??焖偻蛔兊姆椒?/p>
用我們確認(rèn)的三步法快速突變方法,我們引用更快的酶把每一步驟縮短。我們的創(chuàng)新的超高保真的酶有更強的合成能力,讓突變中的引物延伸可以通過2步熱循環(huán)完成。在線性地擴增之后,親代DNA模板在5分鐘內(nèi)被DpnI酶選擇性地降解。我們的新的優(yōu)化過的DpnI酶比典型的DpnI酶快12倍。*,我們的XL10 Gold感受態(tài)細(xì)胞在轉(zhuǎn)化過程中增加了攝入DNA的效率。
適合于高G/C含量的、難于操作的、大的質(zhì)粒
我們的QuikChange誘變試劑盒為在難于擴增的序列和大的質(zhì)粒上進行突變而進行了優(yōu)化??焖倜讣夹g(shù)加速了所有DNA質(zhì)粒類型的突變,包括大到14kb的大質(zhì)粒和那些含有高G/C-富集區(qū)的質(zhì)粒。
QuikChange定點誘變試劑盒
內(nèi)含物:增強創(chuàng)新技術(shù)用于高效率和快遞定點誘變包括QuikChange高保真融合聚合酶,增強Dpn I限制性內(nèi)切酶,10×反應(yīng)緩沖液,dNTPs混合,QuickSolution,QuikChange質(zhì)控質(zhì)粒和質(zhì)控引物,XL10-Gold超級感受態(tài)細(xì)胞,pUC18質(zhì)控質(zhì)粒
量:30次反應(yīng)
?The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours. These QuikChange Lightning kits, like their predecessors, are based on a linear amplification method whereby mutation-containing primers are incorporated but not copied. The protocols of other commercially available kits rely on PCR-based methods, which may result in unwanted second-site errors. The QuikChange Lightning Kit allows you to introduce point mutations, insertions, or deletions twice as fast compared to earlier kit versions, and the QuikChange Lightning Multi Kit delivers mutants up to three times faster than our original multi-site mutagenesis kit—both kits accomplish this without sacrificing the mutagenesis efficiency, reliability and accuracy you have come to expect from the QuikChange name.
?For single site mutagenesis, one kit accommodates both short and long templates (4-14 Kb)
?>80% mutagenesis efficiency for 1 site; >55% mutagenesis efficiency for 3 mutations simultaneously
?QuikChange Lightning Multi Site-Directed Mutagenesis Kit - Academic, 10 Rxn
QuikChange Lightning Multi Site-Directed Mutagenesis Kit contains optimized enzyme formulations that maintain the same accuracy and efficiency of previous QuikChange kits while performing the reaction at up to 50% faster. For academic use only.
200516 Kit,QuikChange XL Site-Directed 30 rxn
200517 Kit,QuikChange XL Site-Directed 10 rxn
200518 Kit,QuikChange Site-Directed 30 rxn
200519 Kit,QuikChange Site-Directed 10 rxn
200521 Kit,QuikChange II XL site-directed 10rx
200522 Kit,QuikChange II XL site-directed 30rx
200523 Kit,QuikChange II site-directed 10 rxn
200524 Kit,QuikChange II site-directed 30 rxn
200555 QuikChange II-E(Electrprtn)StDrctd,10Rx
210518 QuikChange Lightning 10 rxn
210519 QuikChange Lightning 30 rxn
200513 Kit,QuikChange Multi Site-Directed 30rx
200514 Kt,QuikChange MultiSite-Directed,30rx
200515 QuikChange Multi Site-Drctd Mtgns,10 rx
200531 QuikChange MultiSite-DrctdMtgns,10 Rxn,
210513 QC Lightning Multi, 30 Reaction Kit
210514 QC Lightning Multi, 30 rxn Commercial
210515 QC Lightning Multi, 10 Reaction Kit
210516 QC Lightning Multi, 10 rxn Commercial
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